1. What is IDT Primer Resuspension?

IDT (Integrated DNA Technologies) primers are typically shipped in nmol quantities and need to be resuspended in a specific volume of buffer or water to achieve the desired concentration for molecular biology applications.

2. How Does the Calculator Work?

The calculator uses the standard IDT resuspension formula:

Where:

• \( nmol \) — Amount of primer in nanomoles
• 10 — Standard multiplier to achieve 100 µM concentration

Explanation: This calculation results in a 100 µM stock solution when the primer is resuspended in the calculated volume.

3. Importance of Proper Resuspension

Details: Accurate resuspension is crucial for consistent experimental results in PCR, sequencing, and other molecular biology techniques.

4. Using the Calculator

Tips: Enter the nmol value printed on the primer tube. The calculator will determine the appropriate volume of resuspension buffer or nuclease-free water to add.

5. Frequently Asked Questions (FAQ)

Q1: Why multiply by 10?
A: Multiplying the nmol amount by 10 gives the volume (in µL) needed to create a 100 µM stock solution (nmol × 10 = µL for 100 µM).

Q2: What concentration does this produce?
A: This produces a 100 µM stock solution, which is a standard working concentration for many applications.

Q3: Can I use a different final concentration?
A: Yes, but you'll need to adjust the calculation accordingly (Volume = (nmol × 10) × (100/desired concentration in µM)).

Q4: What should I use for resuspension?
A: Typically nuclease-free water or TE buffer, depending on your application and storage requirements.

Q5: How should I store resuspended primers?
A: Store at -20°C for long-term storage. For frequent use, aliquoting is recommended to avoid freeze-thaw cycles.