1. What is Centrifuge Speed Calculation?

The centrifuge speed calculation converts between Relative Centrifugal Force (RCF) and Rotations Per Minute (RPM) based on the rotor radius. This is essential for proper centrifugation protocols in laboratories.

2. How Does the Calculator Work?

The calculator uses the centrifuge speed formula:

Where:

• \( rpm \) — Rotations per minute
• \( rcf \) — Relative centrifugal force in g (gravity)
• \( r \) — Rotor radius in meters
• \( 1.118 \times 10^{-5} \) — Conversion constant

Explanation: The equation shows the inverse relationship between rotor radius and required RPM to achieve a given RCF.

3. Importance of RPM Calculation

Details: Accurate RPM calculation ensures proper separation of samples while preventing damage to samples or equipment from excessive forces.

4. Using the Calculator

Tips: Enter RCF value in g and rotor radius in meters. All values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: Why convert between RCF and RPM?
A: Protocols often specify RCF, but centrifuges display RPM. Conversion ensures proper force is applied regardless of rotor size.

Q2: How do I measure rotor radius?
A: Measure from the center of the rotor to the bottom of the centrifuge tube when in horizontal position.

Q3: What's a typical RCF range?
A: Common ranges are 1,000-20,000g for routine applications, up to 100,000g for ultracentrifugation.

Q4: Does tube position affect calculation?
A: Yes, always use the maximum radius (bottom of tube in horizontal rotors) for safety calculations.

Q5: Are there limitations to this equation?
A: This assumes constant angular velocity. Actual acceleration/deceleration periods may need consideration for sensitive samples.